AN640 – Thermal stability of therapeutic proteins with MicroDSC3-zh

INTRODUCTION

Antibodies are gamma globulin proteins that are found in blood or other bodily fluids of vertebrates, and are used by the immune system to identify and neutralize foreign objects, such as bacteria and viruses. Antibodies can either neutralize targets directly by, for example, binding to an infection-leading part of a pathogen, or recognize an antigen and tag it for attack by other parts of the immune system. DSC allow to test and understand thermally induced unfolding of immunoglobulin.

AN-640

EXPERIMENTAL

The analyzed samples are 10mg/mL aqueous solutions of different immunoglobulins. 800µL of sample were introduced in a µDSC3 Evo batch cell. For compensation, a buffer solution was introduced in a reference cell.

AN-640a

The following thermal profile is programmed:
– Heating between 30°C and 100°C at 1 K.min-1

AN-640b